In order to investigate the retention and elimination of furacillin metabolites (semicarbazide,SEM) in the muscle of T.flavidus following the use of furacilin during the breeding process,the aquaculture pond was treated with 0.50 g/m³ of furacillin.Muscle samples of T.flavidus were then collected at regular intervals.The concentrations of SEM were determined using high-performance liquid chromatography-tandem mass spectrometry,and the data was analyzed by using Excel 2013.The results showed that furacillin was rapidly metabolized to SEM in T.flavidus after dosing.The levels of SEM in the muscles increased rapidly,reaching their highest value after approximately 4 days.The elimination rate of SEM was initially fast,but slowed down over time.Even after 77 days following dosing,the residual amount of SEM in the muscles was still around 2.50 μg/kg,which was above the limit of detection.This suggests that SEM has a prolonged residual time in T.flavidus after the administration of furacilin.Based on these findings,it is strongly recommended to strictly prohibit the use of nitrofurans,such as furacilin,during the breeding process of T.flavidus,in order to ensure the quality and safety of puffer fish.

The aims of the present study were to develop environmental and low cost methods for detection of tetracyclines (TCs) concentrations in the fish by high performance liquid chromatography with chemiluminescence.Luminol chemiluminescence (CL) could be generated by generated active oxygen species of photo-induced nano-titanium dioxide (TiO₂) instead of the traditional chemiluminescent oxidation reagents.Metal ions could catalyze this reaction.Based on the complexation between Fe²⁺ and TCs that remarkably decreased the Fe²⁺-catalyted luminol CL,the determination of TCs was proposed.The results indicated that the limit of quantitation for tetracycline,chloroteracycline and doxycycline were 10.0,30.0 and 10.0 mg/kg,respectively.The proposed method could be applied to the determination of the TCs in the fish,and meet the requirements of residue detection.Nano TiO₂ was used instead of traditional chemiluminescent oxidation reagents to avoid the application of some toxic reagents and chemical reagent pollution,and had the advantages of environmental protection and low price.

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the quantitative determination of mebendazole in aquaculture water.The limit of quantification was 0.5 μg/L.The average recoveries of the three spiked levels ranged from 79.0% to 93.9%.The intra-day and inter-day relative standard deviations (RSDs) were 7.57%-8.27% and 7.43%-8.11%,respectively.The actual application concentrations of 15 μg/L and 50 μg/L were used as the tested concentrations for the elimination experiments of mebendazole in aquaculture water,respectively.The results showed that mebendazole was a photosensitive drug whose photodegradation followed the first-order kinetics.The photolytic degradation rate of mebendazole in outdoor aquaculture water under natural light was more than 80% after 648 h.The elimination half-life of mebendazole was 231.05 h at 15-18℃.At the end of the experiment (648 h),6.4 μg/L of mebendazole was still detected in the high concentration group(50 μg/L).Therefore,the results of this study could provide basic data for the pharmacokinetic study of mebendazole in aquaculture animals.

With the development of economy and population, the environmental pollution, especially water pollution, is becoming more and more serious.This poses a threat to aquatic life and human health.Agricultural solid waste has served as a high potential alternative kind of materials for conventional sorbents because of the low cost, high adsorption capacity, easy modification and simple operation.In this paper, when agricultural solid waste was used as adsorbent, the properties of agricultural waste, adsorption mechanism, influencing factors and adsorption performance of PAHs and OPs in water environment were reviewed.The applications of agricultural wastes as adsorbents were also prospected.